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RAMSEM is a registered semen collection centre.
The purpose of the centre is to collect and freeze sheep and goat semen for the owner and to make available to the small-stock industry.
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ADVANTAGES COLLECTION METHODS SEMEN ANALYSIS FREEZING |
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Advantages of Frozen Semen for the stud breeder.
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Semen Collection by Artificial Vagina.
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Collection of Semen by Electrical Stimulation.
Electrical stimulators with a bipolar rectal electrode that gives a 10-15 Volt output. The ram is restrained in a lateral position. Straightening of the sigmoid flexure extends the penis and the glans penis is grasped and held with a piece of gauze swab. The rectal probe is lubricated and inserted into the rectum to a depth of 15-20cm, taking care to avoid injury. The rectal probe is applied towards the floor of the pelvis and short stimuli are applied with intervals. The glans penis shows congestion and with the correct stimulation semen is collected in a pre-heated and sterile tube.
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Wave motion and Motility:
Assessment of wave motion is the simplest test for motility of fresh undiluted semen. Good wave motion can be seen in the collection glass with the naked eye. Under a microscope a scoring system from 0-5 is assessed for each undiluted sample.
With a good phase contrast microscope equipped with a heated stage, a drop of diluted semen under a cover slip can be examined for progressive motility, percentage of live and dead sperm and also an assessment of the morphology of the sperm. |
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Further tests on semen.
Haemocytometer and colorimeter for more accurate assessment of concentration of semen. Stained smears to examine for sperm morphology and presence of infection.
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Freezing of Semen in straws.
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